Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ)
CAT#: TAB-468MZ
This product is a recombinant human antibody that recognizes NaPi2b (SCL34A2) (Sodium-dependent phosphate transport protein 2b). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37989/1788_A4_7_cr5971b651a2dbe_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66474/1788_E8_17_cr5971fcb00a73f_selected.jpg
Normal Tissue
Figure 3 High expression in lung
Alveolar cells type Alveolar cells type I I Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37989/141847_A_2_4.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (BEWO, EFO-21, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157765-SLC34A2
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SDS-PAGE
Figure 1 Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) in SDS-PAGE
SDS-PAGE analysis of TAB-468MZ in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
SEC-HPLC
Figure 2 Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) in SEC-HPLC
The purity of TAB-468MZ was greater than 95% as determined by SEC-HPLC.
ELISA
Figure 3 Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) in ELISA
ELISA analysis of TAB-468MZ was performed by coating with Recombinant Human SLC34A2 protein , N- GST Tag.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
WB
Figure 4 Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) in WB
Western blot analysis of TAB-468MZ was performed with Recombinant Human SLC34A2 protein , N- GST Tag.
TAB-468MZ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
DB
Figure 5 Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) in DB
Dot blot analysis of TAB-468MZ was performed with Recombinant Human SLC34A2 protein , N- GST Tag.
TAB-468MZ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Specifications
- Host Species
- Human
- Type
- Humanized antibody
- Specificity
- Human NaPi2b (SCL34A2) (Sodium-dependent phosphate transport protein 2b)
- Species Reactivity
- Human
- Applications
- FC, Inhib
- Related Disease
- Ovarian cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SLC34A2; solute carrier family 34 (type II sodium/phosphate cotransporter), member 2; NPTIIb; NAPI-3B; NAPI-IIb; sodium-dependent phosphate transport protein 2B; sodium/phosphate cotransporter 2B; type II sodium-dependent phosphate transporter 3b; solute carrier family 34 (sodium phosphate), member 2
- Gene ID
- 10568
- UniProt ID
- O95436
REVIEWS AND Q&AS
CITATIONS
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Effective in Phosphate Metabolism Studies
Using the TAB-468MZ antibody has significantly enriched our insights into phosphate metabolism pathways. This antibody's specificity for SLC34A2 ensures clean data, necessary for metabolic research. Consistency across experiments is impressive, and Creative Biolabs provides excellent support throughout the purchase and application.
Superior Quality and Reliability
In our cancer biology lab, the TAB-468MZ antibody consistently delivers high-quality results in FC and other assays. Its robust interaction with SLC34A2 enhances our ability to identify potential oncogenic roles of NaPi2b, making it an invaluable tool in our studies.
Valued in Phosphate Transport Studies
As part of our work understanding phosphate transport regulation, the TAB-468MZ antibody provides unmatched specificity for NaPi2b. This has been crucial for elucidating its role in metabolic regulation, with high reproducibility across experiments.
Q&As
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What is the primary application of the SLC34A2 antibody in research?
A: The Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) is mainly used to study the role of sodium-dependent phosphate transport protein 2b in phosphate metabolism. This protein is critical for maintaining phosphate homeostasis, which is crucial for understanding metabolic bone diseases and certain types of cancer, such as ovarian cancer.
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What protein does this antibody specifically bind to?
A: This humanized recombinant antibody is designed to specifically bind to the NaPi2b protein, a member of the solute carrier family 34, which is vital for phosphate ion transport in cells.
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Is this antibody suitable for FC?
A: Yes, the antibody offers high sensitivity and specificity, facilitated by its precise engineering to target SLC34A2 in FC.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-468MZ, RRID: AB_3111959)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| HPAB-M0659-YC-F(E) | Human Anti-SLC34A2 Recombinant Antibody; Fab Fragment (HPAB-M0659-YC-F(E)) | FC, IHC, FuncS | Humanized Fab |
| HPAB-M0281-YC-F(E) | Human Anti-SLC34A2 Recombinant Antibody; Fab Fragment (HPAB-M0281-YC-F(E)) | FuncS | Human Fab |
| HPAB-M0282-YC-F(E) | Human Anti-SLC34A2 Recombinant Antibody; Fab Fragment (HPAB-M0282-YC-F(E)) | FC, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
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| MOB-1661z-S(P) | Recombinant Anti-Human SLC34A2 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| HPAB-M0659-YC-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (HPAB-M0659-YC-S(P)) | FC, IHC, FuncS | Humanized scFv |
| HPAB-M0281-YC-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (HPAB-M0281-YC-S(P)) | FuncS | Human scFv |
| HPAB-M0282-YC-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (HPAB-M0282-YC-S(P)) | FC, FuncS | Human scFv |
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| CAT | Product Name | Application | Type |
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| TAB-H45 | Human Anti-SLC34A2 Recombinant Antibody (TAB-H45) | ELISA, FC, IP, FuncS, IF, Neut, ICC | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-467MZ | Mouse Anti-SLC34A2 Recombinant Antibody (TAB-467MZ) | FC, Inhib | Mouse IgG1 |
| TAB-467MZ-S(P) | Mouse Anti-SLC34A2 Recombinant Antibody; scFv Fragment (TAB-467MZ-S(P)) | SDS-PAGE, IHC, WB | Mouse scFv |
| TAB-467MZ-F(E) | Mouse Anti-SLC34A2 Recombinant Antibody; Fab Fragment (TAB-467MZ-F(E)) | SDS-PAGE, IHC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-468MZ-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (TAB-468MZ-S(P)) | SDS-PAGE, IHC, WB | Humanized scFv |
| TAB-468MZ-F(E) | Human Anti-SLC34A2 Recombinant Antibody; Fab Fragment (TAB-468MZ-F(E)) | SDS-PAGE, IHC, WB | Humanized Fab |
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|---|---|---|---|
| AFC-TAB-H45 | Afuco™ Anti-SLC34A2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H45) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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